| Dehydrated Culture Media - Chromogenic & Fluorogenic Analysis | | | | Put color andfluorescence to work for youin your methods for detection, isolation,enumerationand identification! |
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The value of techniques using fluorogenic and chromogenic substrates in the detection, isolation, enumeration and identification of microorganisms cannot be understated. These techniques involve the use of bright colors and/or fluoresence when reacting with bacterial enzymes or specific bacterial metabolites.
Enzyme tests have the advantage of being easy to perform and they also provide rapid results and information on protein activities coded for by defined genes. | | The target organism contains an enzyme that metabolizes either the MUG or MUP in a way that breaks the bond between the carbohydrate and Methyl-umbelliferon and absorbs the carbohydrate, leaving the Methyl-umbelliferon behind. This Methyl-umbelliferon is visible under 366nm UV light. | | Fluorocult®
EMD’s Fluorocult fluorogenic culture media contain MUG (4-methylumbelliferyl-ß-D-glucuronide) or MUP (4-methylumbelliferyl-phosphate) which reacts with enzymes of the target organisms to create fluorescence.
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Chromocult®
EMD’s Chromocult chromogenic media contain chromogenic substrates which react with enzymes of the target organisms to create color.
 | | The target organism contains an enzyme that metabolizes the colorless chromogenic substrate in a way that breaks the bond between the carbohydrate and indolyl, absorbs the carbohydrate, and leaves behind the indolyl, which oxidizes. This oxidation causes color. Depending on the substrate and test, multiple colors and combinations of colors can be achieved and used to identify different target organisms. |
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